Establishment of human embryonic stem cell lines from frozen-thawed blastocysts using STO cell feeder layers
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چکیده
منابع مشابه
Establishment of human embryonic stem cell lines from frozen-thawed blastocysts using STO cell feeder layers.
BACKGROUND Recently, human embryonic stem (hES) cells have become very important resources for basic research on cell replacement therapy and other medical applications. The purpose of this study was to test whether pluripotent hES cell lines could be successfully derived from frozen-thawed embryos that were destined to be discarded after 5 years in a routine human IVF-embryo transfer programme...
متن کاملDerivation of embryonic stem-cell lines from human blastocysts.
Embryonic stem cells have the unique ability to form all adult cell types. Harnessing this potential may provide a source of cells to replace those that are lost or impaired as a result of disease. Moreover, the derivation of human embryonic stem cells opens a unique window into the study of early human development. At present, approximately 15 human embryonic stem-cell lines are publicly avail...
متن کاملEmbryonic stem cell lines derived from human blastocysts.
Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form troph...
متن کاملEffects of Mouse Strain on Establishment of Embryonic Stem Cell Lines
Purpose: Embryonic stem (ES) cells are derived from the inner cell mass of blastocysts with self-renewal and pluripotency characteristics. These cells have potential for studies of in vitro differentiation, gene function, etc. This study was, therefore, initiated to establish new ES lines and evaluate the effects of strain on ES cell production. Materials and Methods: 3-5 day blastocysts were ...
متن کاملGeneration of cardiomyocytes from new human embryonic stem cell lines derived from poor-quality blastocysts.
Human embryonic stem (hES) cells represent a potential source for cell replacement therapy of many degenerative diseases. Most frequently, hES cell lines are derived from surplus embryos from assisted reproduction cycles, independent of their quality or morphology. Here, we show that hES cell lines can be obtained from poor-quality blastocysts with the same efficiency as that obtained from good...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2004
ISSN: 1460-2350
DOI: 10.1093/humrep/deh102